272
Carbohydrate Analysis
For this reason, lectins are used in blood
banks to identify blood groups. In some
cases, lectin binding is able to discrim-
inate between structures that are oth-
erwise dif±cult to distinguish; examples
include
α
-2-6- and
α
-2-3-linked terminal
sialic acids.
Lectins may be immobilized on Sepha-
rose columns and used to separate, purify,
and type glycoconjugates by a process of
af±nity chromatography. Submicrogram
amounts of glycoconjugates may be differ-
entiated by means of their speci±c binding
to lectins that have been covalently la-
beled with biotin or digoxigenin. A range
of lectins may be used; those bound are
detected by enzyme immunoassay using
streptavidin or a digoxigenin-speci±c anti-
body, respectively, conjugated to alkaline
phosphatase in a similar manner to that
described in Sect. 2.4.1.
See also
Bioorganic Chemistry.
Bibliography
Books and Reviews
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Kennedy, J.F.
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(1994)
Carbohydrate Analysis: A Practical Approach
,
2nd edition, Oxford University Press, London,
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Carbohydrate Analysis by Mod-
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edition, Elsevier Health Sciences, Amster-
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White, C.A. (Ed.) (1996)
Advances in Carbohydrate
Analysis
, JAI Press, London.
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