270
Carbohydrate Analysis
Tab. 4
The specifcity oF some lectins used For characterizing carbohydrates.
Lectin
Systematic name
Abbreviation
Useful speciFcity
a
Example (a–f
designate parts of
±ig. 2)
Orange peel
mushroom
Aleuria aurantia
AAA
Terminal Fucose
(
α
1
6) GlcNAc
(b), (F)
Love lies
bleeding
Amaranthus
caudatus
ACA
Terminal/internal
Gal(
β
1
3)GalNAc(
α
1
)
(d)
Jack bean (con-
canavalin
A)
Canavalia ensiformis
ConA
Terminal/internal
mannose/glucose
(a), (b), (F)
Horse gram
Dolichos biflorus
DBA
Terminal
GalNAc(
α
1
)
Blood type A
Thorn apple
Datura stramonium
DSA
Terminal Gal(
β
1
4)
GlcNAc
Neuraminidase-
treated (b)
or (F)
Terminal
GlcNAc(
β
1
3)
Ser/Thr
(c)
Common
snowdrop
Galanthus nivalis
GNA
Terminal
Man(
α
1
2/3/6)
Man
(a)
AwestAFrican
legume
Griffonia simplicifolia
GS IA
4
,BSIA
4
Terminal
N
-acetyl-
α
-
galactosamine
Blood type A
b
GS IB
4
Terminal
α
-galactose
(e), blood type B
c
Roman snail
Helix pomatia
HPA
Terminal N acetyl-
α
-
galactosamine
Blood type A
Lentil seed
Lens culinaris
LCA
Terminal/internal
α
-
mannose/
α
-glucose
(a), (b), (F)
Winged pea
Lotus tetragonolobus
LTA
Terminal
Fucose(
α
1
6)
GlcNAc
(b), (F)
Maackia tree
Maackia amurensis
MAA
Terminal sialic acid
(
α
2
3) Gal
(d)
Red kidney
bean
Phaseolus vulgaris
PHA
Terminal Gal(
β
1
4)
GlcNAc
Neuraminidase-
treated (b)
Peanut
Arachis hypogaea
PNA
Terminal Gal(
β
1
3)
GalNAc
Neuraminidase-
treated (d)
Castor bean
Ricinus communis
RCA
1
Terminal Gal(
β
1
4)
GlcNAc(
β
1
)
Neuraminidase-
treated (b)
RCA
2
Terminal Gal(
β
1
3)
GalNAc(
α
1
3)
Ser/Thr
Neuraminidase-
treated (d)
Elderberry
Sambucus nigra
SNA
Terminal sialic
acid(
α
2
6)
Gal/GalNAc
(b), (F)
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