254
Carbohydrate Analysis
Tab. 2
Sources and specifcity oF some important enzymes used in carbohydrate analysis.
Enzyme
commission
(EC) number
Common name
Usual source
Useful speciFcity
a
Oxidoreductases
Oxidation of
1.1.1.49
Glucose-6-phosphate
dehydrogenase
Saccharomyces
cerevisiae
Glucose 6-phosphate
1.1.3.4
Glucose oxidase
Aspergillus niger
Glucose
1.1.3.9
Galactose oxidase
Dactylium
dendroides
Terminal galactose residues in
saccharides and
glycoconjugates
Kinases
Phosphorylation of
2.7.1.1
Hexokinase
S. cerevisiae
Hexoses
Hydrolases
Hydrolysis of
3.2.1.1
α
-Amylase
Bacillus
amyloliquefaciens
α
-1,4-Glucans
3.2.1.3
Amyloglucosidase
A. niger
Internal
α
-linked glucose residues
3.2.1.4
Cellulase
Trichoderma viride
β
-1,4-Glucans
3.2.1.14
Chitinase
Streptomyces sp.
β
-1,4-
N
-Acetylglucosamine links
in chitin
3.2.1.17
Lysozyme
Hen egg white
GlcNAc(
β
1
4)-
N
-
acetylmuramic acids in
peptidoglycans
3.2.1.18
Neuraminidase
Clostridium
perfringens
Terminal
α
2-linked
N
-acetyl/
N
-glycolyl neuraminic
acids
3.2.1.18
Neuraminidase
Vibrio cholerae
Terminal
α
2-linked
N
-acetyl/
O
-acetyl neuraminic
acids
3.2.1.20
α
-Glucosidase
S. cerevisiae
Terminal
α
-linked glucose in di-
and trisaccharides
3.2.1.21
β
-Glucosidase
Sweet almonds
β
-Linked hexose in disaccharides
3.2.1.22
α
-Galactosidase
Green coFFee beans
Terminal
α
-linked galactose in
glycans
3.2.1.23
β
-Galactosidase
Escherichia coli
β
-Linked galactose in
disaccharides
3.2.1.23
β
-Galactosidase
Bovine testes
Terminal
β
-linked galactose in
glycans
3.2.1.24
α
-Mannosidase
Jack bean
Terminal
α
-linked mannose in
glycans
3.2.1.25
β
-Mannosidase
A. niger
Terminal
β
-1,4-linked mannose in
glycans
3.2.1.26
Invertase
S. cerevisiae
Terminal
β
-linked Fructose in
oligosaccharides
3.2.1.30
N
-Acetyl-
β
-
glucosaminidase
Diplococcus
pneumoniae
Terminal
β
-linked GlcNAc in
glycans
3.2.1.31
β
-Glucuronidase
E. coli
Terminal
β
-linked glucuronic acid
in polysaccharides
3.2.1.35
Hyaluronidase
Ovine testes
GlcNAc(
β
1
4)-glucuronic
acids in hyaluronates
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