243
Carbohydrate Analysis
Martin F. Chaplin
South Bank University, London, UK
1
Outlines of Methodology
246
1.1
Introduction
246
1.2
Monosaccharides
249
1.3
Oligosaccharides
250
1.4
Polysaccharides
250
1.5
Proteoglycans
250
1.6
Glycoproteins
251
1.7
Glycolipids
253
2
Key Methods
253
2.1
The Use of Enzymes in Analysis
253
2.2
Chromatography
255
2.3
Determination of Size and Conformation
259
2.3.1
Light Scattering
259
2.3.2
Size-exclusion Chromatography
260
2.3.3
Field Flow Fractionation
260
2.3.4
Mass Spectrometry (MS)
261
2.3.5
NMR Spectrometry
263
2.3.6
Molecular Modeling
266
2.4
Linkage Analysis
266
2.4.1
Glycan Detection
266
2.4.2
Glycan Release and the Use of Enzymes
266
2.4.3
Methylation Analysis
266
2.4.4
Mass Spectrometry
268
2.4.5
NMR Spectroscopy
268
2.5
Use of Lectins
271
Encyclopedia of Molecular Cell Biology and Molecular Medicine, 2nd Edition
. Edited by Robert A. Meyers.
Copyright
2004 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
ISBN: 3-527-30544-0
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