76
Body Expression Map of Human Genome
Alignment with Large-scale Genomic Sequences
84
Oligonucleotide Probe Design
84
Keywords
Gene
A part of genome, which is coded for proteins or templates of many functional RNAs.
Genome
A set of genes, which are required for a particular organism.
Gene Expression
A process in which DNA of a gene is transcribed into RNA, and then translated into
protein.
Alignment Algorithm
A computer algorithm used to compare multiple nucleotide or amino acid sequences.
SNP Classifcation
A classiFcation that is necessary because current single nucleotide polymorphism
(SNP) data tend to have errors.
EST Orientation
EST sequences can be aligned onto genomic DNA according to which strand of the
genomic DNA is transcribed.
Oligomer Design
Oligo-nucleotide whose sequence is unique within the particular genome is useful for
various purposes, for example, designing of DNA-chip.
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One of the major objectives of the Human Genome Project (HGP) was to list the
entire set of genes and their functions together with control mechanisms written in
the human genome. Thus, genome-wide mRNA/cDNA analyses were conducted at
about the same time as the major research topic of the Human Genome Project. The
Frst approach was to compile a complete catalog of genes written in the genome of
particular organisms, such as human or mouse, through a random collection of ex-
pressed cDNA sequences (expressed-sequence tags, ESTs). The second approach has
more focus on the status of expressed genes through the acquisition of expression
proFles of mRNA/cDNA (body mapping) isolated from a particular cell group, tissue,
or organ. Much of the EST approach, such as the gene cataloguing project,
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