Bacterial Cell Culture Methods
521
needle. This needle can touch an iso-
lated bacterial colony and is then drawn
through
the
12
compartments,
inocu-
lating the test media. Fifteen standard
tests are performed. After 18 to 24 h
of incubation the color changes that oc-
cur in each of the compartments are
recorded. Interpretation of the results is
determined using a ±ve-digit code ob-
tained from the results. A differential
chart, supplied by the manufacturer, or
a computer-based program, (ENCISE) En-
terobacteriaceae
numerical
coding
and
identi±cation system for Enterotube can
be used.
5.2
Cultural Characteristics
In addition to the morphological and
biochemical characters, it is essential to
record the appearance of bacterial colonies
under de±ned conditions, for example, on
speci±c media as agar streak plates, gelatin
stabs, and in broth culture. The shape and
structure of colonies can be described as
shown in the ±gure below:
Colonies on plates
Form
Punctiform
Circular
Filamentous
Irregular
Rhizoidal
Spindle
Flat
Raised
Convex
Pulvinate
Umbonate
Entire
Undulate
Lobate
Erose
Filamentous
Curled
Elevation
Margin
Agar slopes
Gelatin stab
Filoform
Echinulate
Beaded
Effuse
Aborescent
Rhizoid
Filoform
Beaded
Papillate
Vilous
Aborescent
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