Bacterial Cell Culture Methods
Biochemical Examination of Anaerobic Bacteria
Rapid Testing Methods
Cultural Characteristics
Appendix 1 Sources of Media and Supplies
Bacterial Isolation
This is he separation from a sample of one or more bacterial species. The basis of the
separation is ability of these isolates to grown under selective conditions. This
selectivity can be physical, chemical or biological in nature.
Bacterial Culture
A particular strain or kind of bacteria growing in a laboratory medium. It is usually
assumed that the bacteria is growing in pure culture, without contamination.
The observation of bacteria requires the useofmicroscopy.Anumberofmethodscan
be used to enhance the appearance of bacteria under the microscope. Simple or
complex stains are widely used.
Population Estimation
Determining the numbers of bacteria present in a sample is often required. A number
of techniques can be used including direct microscopic counts, dilution plate counts,
absorbance and most probable numbers. Each can give useful estimates of population
increases and hence growth rates.
Biochemical Characterization
Microscopic observations rarely allow bacterial identiFcation to the genus level. The
metabolic activities and other characteristics of the bacteria are also required.
Biochemical characterization of the bacteria assits in the identiFcation of the organism.
This chapter provides essential information to help the researches in the successful
culturing of a wide range of bacteria. It includes guidance on the isolation, culturing,
microscopic examination, characterization and identiFcation of bacteria.
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