503
Bacterial Cell Culture Methods
Andrew S. Ball
Department of Biological Sciences, University of Essex, Colchester, UK
1
Cultivation of Bacteria
505
1.1
Introduction
505
1.2
Cultures
505
1.3
Culture Incubations
506
1.4
Types of Media Used for Growing Selected Bacteria
506
2
Methods of Bacterial Isolation
507
2.1
Sterilization
507
2.2
PuriFcation of Bacteria from Contaminated Cultures
507
2.3
Isolation of Bacteria from Natural Sources
508
2.4
Use of Selective Media
508
2.5
Methods for Anaerobic Cultures
509
3
Microscopic Examination of Bacteria
510
3.1
Introduction
510
3.2
The Examination of Living Unstained Bacteria
510
3.3
The Measurement of Bacteria
510
3.4
Methods of Staining Bacteria
510
4
Estimation of Population Sizes of Bacteria
512
4.1
Bacterial Biomass Measurement
512
4.2
Turbidometric Estimations
512
4.3
Theory of Bacterial Growth
513
4.4
Counting Methods
513
5
Characterization of Bacteria Using Biochemical and Cultural Tests
516
5.1
Introduction
516
5.1.1
Minimal Growth Requirements and Substrate Utilization
517
5.1.2
Detection of Metabolic End Products
517
Encyclopedia of Molecular Cell Biology and Molecular Medicine, 2nd Edition
. Edited by Robert A. Meyers.
Copyright
2004 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
ISBN: 3-527-30543-2
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