468
Autoantibodies and Autoimmunity
Tab. 2
Examples of subcellular structures and domains recognized by autoantibodies.
Autoantibody
Molecular specifcity
Subcellular structure
Nuclear components
Antichromatin
Nucleosomal and subnucleosomal
complexes of histones and DNA
Chromatin
Anti-nuclear pore
210 kDA glycoprotein (gp210)
Nuclear pore
Antilamin
Nuclear lamins A, B, C
Nuclear lamina
Anticentromere
Centromere proteins (CENP) A, B, C,
F
Centromere
Anti-p80 coilin
p80-coilin (80 kDa protein)
Coiled body
Anti-PIKA
p23–25 kDa proteins
Polymorphic interphase
karyosomal association
(PIKA)
Anti-NuMA
238 kDa protein
Mitotic spindle apparatus
Nucleolar components
Anti±brillarin
34 kDa ±brillarin
Dense ±brillar component of
nucleolus
Anti-RNA polymerase 1
RNA polymerase 1
Fibrillar center of nucleolus
Anti-Pm-Scl
75 and 100 kDa proteins of Pm-Scl
complex
Granular component of
nucleolus
Anti-NOR 90
90 kDa doublet of (human) upstream
binding factor (hUBF)
Nucleolar organizer region
(NOR)
Cytosolic components
Antimitochondria
Pyruvate dehydrogenase complex
Mitochondria
Antiribosome
Ribosomal P-proteins (P
0
,P
1
,P
2
)
Ribosomes
Anti-Golgi
95 and 160 kDa golgins
Golgi apparatus
Antiendosome
180 kDa protein
Early endosomes
Antimicrosomal
Cytochrome P450 superfamily
Microsomes
cANCA
Serine proteinase (proteinase 3)
Lysosomes
Antimidbody
38 kDa protein
Midbody
Anticentrosome/centriole
Pericentrin (48 kDa)
Centrosome/centriole
Notes
: NuMA: nuclear mitotic apparatus; Pm-Scl: polymyositis-scleroderma; cANCA: cytoplasmic
antineutrophil cytoplasmic antibody.
that
react
with
subcellular
structures
other than the nucleus. Figure 4 shows
the IIF patterns produced by some of
these autoantibodies. Prior knowledge of
subcellular organelles and their relative
cellular distribution was instrumental in
identifying the structures recognized by
these and other autoantibodies. In turn,
autoantibodies, by virtue of their reactivity
with individual autoantigens, have allowed
cell and molecular biologists an insight
into the molecular constituents of these
subcellular organelles.
Comparative studies using human au-
toantibodies,
and
nonhuman
autoanti-
bodies raised by immunization against
speci±c cellular proteins, can be useful
in determining the cellular distribution
of the speci±c protein. This is achieved
by using antihuman antibodies labeled
with one chromophore and antibodies
speci±c for the nonhuman antibody that
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