415
Antitumor Agents: Taxol and
Taxanes – Production by Yew
Cell Culture
Arthur G. Fett-Neto,
1
Hideki Aoyagi,
2
Hideo Tanaka
2
,andFrankD
iCosmo
3
1
Universidade Federal Do Rio Grande Do Sul, Porto Alegre, Brazil
2
University of Tsukuba, Tsukuba, Ibaraki, Japan
3
University of Toronto, Toronto, ON, Canada
1
Plant Material Selection and Preparation
417
1.1
Type and Source of Explant
417
1.2
Sterilization Procedure
418
1.3
Explant Size and Final Preparation
418
2
Establishment of Callus and Cell Cultures
418
2.1
General Procedure
418
2.2
Medium Optimization
419
3A
n
a
l
y
s
e
s
o
f
T
a
x
o
l
and Taxane Production
422
3.1
Extraction Procedure
422
3.2
Separation, Quanti±cation and Identi±cation
423
4T
a
x
o
l
Biosynthesis
424
5
General Characteristics of Taxol
Yield and Growth
in
Taxus
Cell Culture
426
6
Techniques to Improve Taxol
Yield in Cell Culture
429
6.1
Precursor and Sugar Feeding
429
6.2
Cell Line Selection
430
6.3
Light and Temperature
430
6.4
Elicitation and Metabolic Inhibitors
431
6.5
Gas Composition
432
6.6
Protoplast Cultures
432
6.7
Culture System
435
7
Scale-up of Taxus Cell Cultures
435
Encyclopedia of Molecular Cell Biology and Molecular Medicine, 2nd Edition
. Edited by Robert A. Meyers.
Copyright
2004 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
ISBN: 3-527-30543-2
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