209
Animal Biotechnology and
Modeling
Carl A. Pinkert, Michael H. Irwin, and Robert L. Howell
School of Medicine and Dentistry, University of Rochester, 601 Elmwood Ave.,
Rochester, NY 14642, USA
1
Introduction
211
1.1
Brief History and DeFnition of Transgenic Animals
211
1.2
Applications of Transgenic Animals
212
1.3
Societal and Ethical Considerations
214
2
Production of Transgenic Laboratory Animals
215
2.1
Choice of Animals
215
2.2
Gain-of-function, Loss-of-function and Conditional Modeling
216
2.3
Methodologies
216
2.3.1
DNA Microinjection
216
2.3.2
Retroviral Introduction of Transgenes
218
2.3.3
Embryonic Stem Cell Technology
219
2.3.4
Nuclear Transfer (Cloning) Methods
220
2.3.5
Other Methodologies
221
2.4
Other Laboratory Animal Models
222
3
Production of Transgenic Domestic Animals
223
3.1
Overview
223
3.2
Methodology
224
3.2.1
DNA Microinjection
224
3.2.2
Retroviral Infection
225
3.2.3
Nuclear Transfer
225
3.3
Traits Affecting Domestic Animal Productivity
227
3.4
Domestic Animals as Bioreactors
228
3.5
Examples from Domestic and Miniature Pigs
228
4
Analysis of Transgene Integration and Expression
231
4.1
Transgene Integration
231
Encyclopedia of Molecular Cell Biology and Molecular Medicine, 2nd Edition
. Edited by Robert A. Meyers.
Copyright
2004 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
ISBN: 3-527-30543-2
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