55
Circular Dichroism in Protein
Analysis
Zhijing Dang and Jonathan D. Hirst
School of Chemistry, University of Nottingham, University Park, Nottingham, UK
1
Chirality and Polarized Light
57
2
Electronic Circular Dichroism
58
2.1
Far Ultraviolet
59
2.2
Near Ultraviolet
61
3
Vibrational Circular Dichroism
61
4
Estimation of Secondary Structure Content
62
5
Experimental Aspects
63
5.1
Instrumentation
63
5.2
Measurements
64
5.3
Solvents and Temperature Effects
65
5.4
Stopped-flow
65
5.5
Synchrotron Radiation
66
5.6
Fast Time-resolved Techniques
66
6
Spectral Characteristics of Elements of Protein Structures
67
6.1
α
-helix
67
6.2
3
10
-helix
68
6.3
β
-sheet
68
6.4
β
-turn
69
6.5
Random Coil
69
6.6
Polyproline Helices
70
6.7
Extrinsic Chromophores
70
6.8
Membrane Proteins
70
7
Conclusion and Outlook
71
Encyclopedia of Molecular Cell Biology and Molecular Medicine, 2nd Edition
. Edited by Robert A. Meyers.
Copyright
2004 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
ISBN: 3-527-30545-9
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