18
Chromosome, Microdissection and Microcloning
6
Applications of Chromosome and Tissue Microdissection
43
6.1
Direct Analysis of the PCR Product of Microdissected
Chromosome Fragments
43
6.2
Recombinant DNA Libraries Generated from Microdissected
Chromosome Fragments
44
6.3
Isolation of Transcribed Sequences Using Microdissected
DNA as a Probe
44
6.4
Study of Chromosome Abnormalities in Cancer Cells
45
6.5
Investigation of Other Specialized Chromosomal Structures
46
6.6
Gene Transfer Using Chromosome Fragments
47
6.7
Coupling Microdissection with Microarrays and Proteomics
Technologies (DNA/Protein Microchips)
48
6.8
Incorporation of Microdissection Techniques in Modern Pathology
50
7
Summary
52
Bibliography
52
Books and Reviews
52
Primary Literature
52
Keywords
BAC
Bacterial artiĀ±cial chromosome based cloning vector capable of accommodating large
fragments of genomic DNA.
Chromosomal Band
Variation in the staining of DNA due to differential binding of dyes along the length of
the chromosome.
Chromosome
A self-replicating genetic structure that contains the cellular DNA representing the
linear array of genes.
Chromosome Fragment
A small portion of chromosomal DNA obtained by means of physical excision using
microdissection.
Gene Mapping
Determining the relative position of one or more genes on chromosomes.
Karyotype
A photomicrograph of individual chromosomes arranged in a standard format
showing the number, size, and shape of each chromosome.
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