Chromosome Organization within the Nucleus
Books and Reviews
Type of Brownian motion in which an object undergoes random, thermally driven
movements, just as in free diffusion, except that the object is prevented from diffusing
outside a limited region of space. Chromatin motion during interphase appears to be
dominated by this type of motion.
FISH (Fluorescence In Situ Hybridization)
A method of visualizing the position of individual chromosome loci during interphase
by hybridization with an exogenous DNA probe that is either itself ﬂuorescently
labeled, or that contains an epitope that can be detected with ﬂuorescent probes.
Regions of chromatin containing mostly simple-sequence repeated DNA that is highly
compacted during interphase. Most heterochromatin is transcriptionally inactive.
The double bilayer that surrounds the nucleus. The ER derived from endoplasmic
reticulum, is organized by a protein-based network containing nuclear lamins, and is
penetrated by pores called
nuclear pore complexes
, which allow import and export of
proteins and RNA molecules.
A putative internal protein-based network that criss-crosses the nuclear lumen and
may help organize chromatin.
The polarized organization of chromosomes in the nucleus, with centromeres
clustered at one end of the nucleus and telomeres at the other. The Rabl conFguration
is a remnant of the anaphase chromosome conFguration.
The interphase nucleus provides the structural context for chromosome biology,
including gene expression and recombination. The organization of chromosomes
within the nucleus is not random. Instead, chromosomes are organized by speciFc
constraints, including interactions of speciFc loci with the nuclear envelope. This