2
Chromosome Organization within the Nucleus
7
Conclusions
11
Acknowledgments
11
Bibliography
11
Books and Reviews
11
Primary Literature
11
Keywords
Constrained Diffusion
Type of Brownian motion in which an object undergoes random, thermally driven
movements, just as in free diffusion, except that the object is prevented from diffusing
outside a limited region of space. Chromatin motion during interphase appears to be
dominated by this type of motion.
FISH (Fluorescence In Situ Hybridization)
A method of visualizing the position of individual chromosome loci during interphase
by hybridization with an exogenous DNA probe that is either itself fluorescently
labeled, or that contains an epitope that can be detected with fluorescent probes.
Heterochromatin
Regions of chromatin containing mostly simple-sequence repeated DNA that is highly
compacted during interphase. Most heterochromatin is transcriptionally inactive.
Nuclear Envelope
The double bilayer that surrounds the nucleus. The ER derived from endoplasmic
reticulum, is organized by a protein-based network containing nuclear lamins, and is
penetrated by pores called
nuclear pore complexes
, which allow import and export of
proteins and RNA molecules.
Nuclear Matrix
A putative internal protein-based network that criss-crosses the nuclear lumen and
may help organize chromatin.
Rabl Con±guration
The polarized organization of chromosomes in the nucleus, with centromeres
clustered at one end of the nucleus and telomeres at the other. The Rabl conFguration
is a remnant of the anaphase chromosome conFguration.
¥
The interphase nucleus provides the structural context for chromosome biology,
including gene expression and recombination. The organization of chromosomes
within the nucleus is not random. Instead, chromosomes are organized by speciFc
constraints, including interactions of speciFc loci with the nuclear envelope. This
previous page 1322 Encyclopedia of Molecular Cell Biology and Molecular Medicine read online next page 1324 Encyclopedia of Molecular Cell Biology and Molecular Medicine read online Home Toggle text on/off