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Chlamydomonas
Keywords
BAC
Bacterial artifcial chromosome, large cloned segment oF chromosomal DNA that can
be propagated in bacteria.
Cosmid
A plasmid that contains phage lambda cos sites and thereFore can be packaged
in vitro
into phage coats.
DNA Complexity
The total length oF diFFerent DNA sequences contained within a given DNA
preparation; the DNA complexity is usually given in kilobase pairs (kb; 1 kb
=
1000
base pairs).
EST
Expressed sequence tag, sequence oF gene Fragment obtained by high-throughput
sequencing.
Intron
A segment oF DNA within a gene that is transcribed but removed From the RNA
transcript by splicing together the flanking coding sequences (exons).
Linkage Group
All loci that are linked together by genetic criteria, as an entity, and correspond to a
chromosome.
Open Reading Frame
A DNA sequence that consists oF a series oF triplets (codons) coding For amino acids
and potentially encodes a protein.
Splicing
Process oF removing introns From a precursor RNA and joining oF flanking exons.
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The highly diversifed, polyphyletic genus Chlamydomonas comprises more than
450 species oF unicellular flagellated photosynthetic algae. These organisms are espe-
cially attractive For several areas oF research in cell and molecular biology because oF
their small size, Fast growth rate, and short sexual cycle. However, only a Few species
have been actively used For molecular genetic studies. They include the heterothallic
species
C. reinhardtii
and
C. smithii
, which are in the line oF descent leading to the
multicellular species
Volvox
; the sibling species pair
C. eugametos
and
C. moewusii
,
both very distinct From
C. reinhardtii
; and the homothallic species
C. monoica
.Th
is
article Focuses on
C. reinhardtii
because this alga has emerged as the organism oF
choice For most investigations.
Chlamydomonas eugametos
and
C. moewusii
have also
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